(317) A 3D Printed Device for Electrophysiological Measurement of Myometrial Smooth Muscle Cell Contractions In vitro

Objective: Current methods for determining uterine cell contractions using in vitro cell models are insufficient for preclinical drug trials as the 2D environment do not mimic intercellular interactions. To address this, we developed a non-invasive and functional model for recording uterine muscle contractions using a 3D-printed device incorporated with an isometric force transducer and measured real-time contractions and assessed the device's functionality in vitro.

Study Design: Myometrial cells were 3D printed using a combination of GelMA (gelatin methacryloyl) and collagen, along with an isometric force transducer to enable real-time recording of uterine muscle contractions. Cell viability within the different biocompatible materials (GelMA and Collagen) was assessed using live-dead staining. In vitro, experiments were conducted with the device to evaluate myometrial cell contractility by stimulating the cells with acetylcholine at a concentration of 1μM.

Results: The 3D-printed device model provided a suitable platform for culturing uterine muscle cells and mimicking their physiological environment. Myometrial cells were viable (green) in biocompatible materials like GelMA and collagen-1 ( Fig 1A). With a 3D printed device ( Fig 1B), we could record the cyclical contraction and relaxation of the 3D myometrial cell culture, using an isometric transducer and LabChart software (Fig 1B). The device successfully replicated the architectural and contractile properties of the native myometrial tissue, providing a conducive environment for culturing uterine muscle cells.

Conclusion: This non-invasive tool presents a promising avenue for studying uterine physiology and conducting preclinical trials, such as uterotonics, tocolytics, and treatments for postpartum bleeding. The tool allows real-time monitoring of drug responses to uterine contractions, enabling researchers to study drug efficacy and safety more effectively. Further research and validation will contribute to obstetric advancements, benefitting maternal and fetal healthcare and the development of new therapeutics.